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WOUND HEALING, MICROCIRCULATION AND ISCHEMIA, FLAP SURGERY

WOUND HEALING
- Hypertrophic scars and keloids. Histological and clinical follow-up. Clinical trial with g interferon, local radiotherapy and chemotherapy associated with surgical excision (Prof Gabbiani).
- Wound healing physiology and the role of apoptosis in the remodelling phase of the granulation tissue to a scar has been studied on a wound rat model. Induction of myofibroblast apoptosis by wound coverage with non-vascularized (skin grafts) or vascularized tissue (skin, muscle or fat flaps) (Prof Gabbiani).
- Wound healing and ischemia: description of a new animal model of ischemic wounds in rat. Correlation between wound healing delay, decrease in wound contraction and a significant delay in myofibroblast appearance in the wound as well as a decrease in the absolute number of myofibroblasts (Prof Gabbiani, Prof Pepper).

MICROCIRCULATION AND ISCHEMIA
1)SURVIVAL OF ISCHEMIC TISSUE
- Experimental study on physiology, histology of tissue ischemia and the onset of irreversible necrosis on an ischemic skin flap model in rats. Expression of endogenous VEGF and its tyrosine kinase receptors has been measured in the flap by immunohistochemistry to demonstrate the presence of angiogenesis. VEGF and bFGF were applied under the flap to stimulate in situ angiogenesis using an ex vivo gene therapy approach with transfected cells placed under the flap in semi-permeable capsules to increase flap survival (Prof Pepper, Prof Aebischer).
- Dynamic study of cutaneous blood flow under ischemic condition has been carried out using laser-doppler analysis and microangiography. Inhibition of microvascular thrombosis by parenteral administration of various coagulation inhibitors (inactivated factor VII, rNAPc2) to decrease tissue necrosis under ischemic condition has been successfully tested (Prof Pepper). The efficacy of intermittent hypoxia training (IHT) as a hypoxic preconditioning procedure has been tested. IHT consisted of 20 daily sessions of alternative exposure of hypoxic gas mixture and ambient air. A significant decrease of PO2 during IHT sessions, with a tendency to disappear after 4 weeks of IHT, has been demonstrated. Preconditioning with IHT led to a significant increase of overall flap survival. Analysis of mRNA-expression of vascular endothelial growth factor (VEGF) and its receptors in skin, as well as in the flap, after a 4 week course of IHT to compare rats preconditioned with IHT versus a control group, did not reveal a significant difference.

2) MECHANISMS OF SECONDARY AGGRAVATION OF BURN LESIONS Development of an animal model of burn lesion in rats. Analysis of the microcirculation around the lesion by laser-doppler and microangiography to demonstrate the secondary aggravation of the burn lesion. Administration of coagulation inhibitor (rNAPc2) to stop the mechanism of secondary burn extension(Prof Pepper)

FLAP SURGERYARTERIALIZED-VENOUS FLAPS
Experimental research on new types of flaps: the arterialized-venous flap. Development of the animal model (rabbit ear), study of the mechanism of survival and the role of neovascularization in this survival. Anatomical classification of the various types of arterialized-venous flap; study of the maximal surface of flap survival based on the anatomy of the vascular venous tree within the flap.

2) ANATOMICAL STUDY
Description of a new flap ; the serratus anterior musculo-cutaneous . Based on anatomical dissection, description of the cutaneous perforator vessels reaching the skin paddle (Prof Fasel ).

Group's publications

Locating perforator vessels by dynamic infrared imaging and flow Doppler with no thermal cold challenge.
ANNALS OF PLASTIC SURGERY
2011 vol. 67(2) pp. 143-146
TENORIO X, MAHAJAN AL, ELIAS B, VAN RIEMPST JS, WETTSTEIN R, HARDER Y, PITTET-CUENOD B

A facial inflammatory myofibroblastic tumour in a 6-year-old girl: plastic surgery lessons from a rare case.
JOURNAL OF CRANIO-MAXILLO-FACIAL SURGERY : OFFICIAL PUBLICATION OF THE EUROPEAN ASSOCIATION FOR CRANIO-MAXILLO-FACIAL SURGERY
2011 vol. 39(2) pp. 141-144
MODARRESSI A, PIETRAMAGGIORI G, LACROIX S, RILLIET B, PITTET-CUENOD B

Superior pedicle breast reduction for hypertrophy with massive ptosis.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
2011 vol. 64(4) pp. 500-507
WETTSTEIN R, CHRISTOFIDES E, PITTET-CUENOD B, PSARAS G, HARDER Y

Nonactivated versus thrombin-activated platelets on wound healing and fibroblast-to-myofibroblast differentiation in vivo and in vitro.
PLASTIC AND RECONSTRUCTIVE SURGERY
2011 vol. 129(1) pp. 46-54
SCHERER SS, TOBALEM M, VIGATO E, HEIT Y, MODARRESSI A, HINZ B, PITTET-CUENOD B, PIETRAMAGGIORI G

Frostbite complicating therapeutic surface cooling after heat stroke.
INTENSIVE CARE MEDICINE
2010 vol. 36(9) pp. 1614-1615
TOBALEM M, MODARRESSI A, ELIAS B, HARDER Y, PITTET-CUENOD B

Hypoxia impairs skin myofibroblast differentiation and function.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
2010 vol. 130(12) pp. 2818-2827
MODARRESSI A, PIETRAMOGGIORI G, GODBOUT C, VIGATO E, PITTET B, HINZ B

[Orbitotemporal facial involvement in type 1 neurofibromatosis (NF1)].
NEURO-CHIRURGIE
2010 vol. 56(2-3) pp. 257-270
RILLIET B, PITTET-CUENOD B, MONTANDON D, NARATA AP, DE RIBEAUPIERRE S, SCHILS F, BOSCHERINI D, DI ROCCO C, DUCREY N

Body taping for contour surgery.
AESTHETIC PLASTIC SURGERY
2009 vol. 33(3) pp. 324-326
KALBERMATTEN DF, WETTSTEIN R, ERBA P, RIEGER UM, PIERER G, RAFFOUL W

[About 10 cases of Launois-Bensaude disease].
ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE
2009 vol. 54(2) pp. 135-145
PAUCHOT J, GOLAY A, GUMENER R, MONTANDON D, PITTET-CUENOD B

Osseous alterations at the interface of hydrogel expanders and underlying bone.
JOURNAL OF CRANIO-MAXILLO-FACIAL SURGERY : OFFICIAL PUBLICATION OF THE EUROPEAN ASSOCIATION FOR CRANIO-MAXILLO-FACIAL SURGERY
2009 vol. 37(5) pp. 258-262
STUEHMER C, RUCKER M, SCHUMANN P, BORMANN KH, HARDER Y, SINIKOVIC B, GELLRICH NC

Improved cutaneous healing in diabetic mice exposed to healthy peripheral circulation.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
2009 vol. 129(9) pp. 2265-2274
PIETRAMOGGIORI G, SCHERER SS, ALPEROVICH M, CHEN B, ORGILL DP, WAGERS AF

Early detection of flap failure using a new thermographic device.
JOURNAL OF SURGICAL RESEARCH
2009 vol. 151(1) pp. 15-21
TENORIO X, MAHAJAN AL, WETTSTEIN R, HARDER Y, PAWLOVSKI M, PITTET B

Erythropoietin reduces necrosis in critically ischemic myocutaneous tissue by protecting nutritive perfusion in a dose-dependent manner.
SURGERY
2009 vol. 145(4) pp. 372-383
HARDER Y, AMON M, SCHRAMM R, CONTALDO C, METZKOW E, MATZEN A, RUCKER M, VOLLMAR B, MENGER MD

Hemiface rhytidectomy.
AESTHETIC PLASTIC SURGERY
2008 vol. 32(2) pp. 227-233
KALBERMATTEN DF, ERBA P, WETTSTEIN R, PIERER G

Erythropoietin protects critically perfused flap tissue.
ANNALS OF SURGERY
2008 vol. 248(6) pp. 919-929
REZAEIAN F, WETTSTEIN R, AMON M, SCHEUER C, SCHRAMM R, MENGER MD, PITTET-CUENOD B, HARDER Y

Microvascular experimental evidence on the relative significance of restoring oxygen carrying capacity vs. blood viscosity in shock resuscitation.
BIOCHIMICA ET BIOPHYSICA ACTA : INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND BIOPHYSICS.
2008 vol. 1784(10) pp. 1421-1427
SALAZAR VÁZQUEZ BY, WETTSTEIN R, CABRALES P, TSAI AG, INTAGLIETTA M

Monocyte chemoattractant protein-1 secreted by adipose tissue induces direct lipid accumulation in hepatocytes.
HEPATOLOGY
2008 vol. 48(3) pp. 799-807
CLEMENT S, JUGE-AUBRY CRISTIANA, SGROI A, CONZELMAN S, PAZIENZA VALERIO, PITTET-CUENOD B, MEIER CA, NEGRO F

Hypoxic preconditioning increases skin oxygenation and viability but does not alter VEGF expression or vascular density.
HIGH ALTITUDE MEDICINE & BIOLOGY
2008 vol. 9(1) pp. 76-88
SCHLAUDRAFF KU, PEPPER MS, TKATCHOUK EN, EHRENBURG I, ALIZADEH N, MONTANDON D, PITTET B

Ischemia-induced up-regulation of heme oxygenase-1 protects from apoptotic cell death and tissue necrosis.
JOURNAL OF SURGICAL RESEARCH
2008 vol. 150(2) pp. 293-303
HARDER Y, AMON M, SCHRAMM R, RUCKER M, SCHEUER C, PITTET B, ERNI D, MENGER MD

Mixed arterio-venous insufficiency in random skin flaps in the rat: is the application of medicinal leeches beneficial?
JOURNAL OF SURGICAL RESEARCH
2008 vol. 150(1) pp. 85-91
SCHLAUDRAFF KU, BEZZOLA T, MONTANDON D, PEPPER MS, PITTET B

Optimizing the arterialized venous flap.
PLASTIC AND RECONSTRUCTIVE SURGERY
2008 vol. 122(6) pp. 1681-1689
PITTET-CUENOD B, QUINODOZ P, ALIZADEH N, SCHLAUDRAFF KU, MAHAJAN AL

An old dream revitalised: preconditioning strategies to protect surgical flaps from critical ischaemia and ischaemia-reperfusion injury.
THE JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGICAL NURSING : OFFICIAL ORGAN OF THE AMERICAN SOCIETY OF PLASTIC AND RECONSTRUCTIVE SURGICAL NURSES
2008 vol. 61(5) pp. 503-511
HARDER Y AND AL.

Review of 197 consecutive free flap reconstructions in the lower extremity.
THE JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGICAL NURSING : OFFICIAL ORGAN OF THE AMERICAN SOCIETY OF PLASTIC AND RECONSTRUCTIVE SURGICAL NURSES
2008 vol. 61(7) pp. 772-776
WETTSTEIN R, SCHURCH R, BANIC A, ERNI D, HARDER Y

[Letters to the editor].
ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE
2007 vol. 52(6) pp. 628-629
PITTET-CUENOD B

Mastectomie avec préservation cutanée et reconstruction immédiate
BULLETIN DU CANCER : JOURNAL DE LA SOCIETE FRANCAISE DU CANCER
2007 vol. 94(9) pp. 833-840
VLASTOS GEORGES, ELIAS B, MEYER OLNA, HARDER YVES, SCHÄFER PIERRE, PITTET BRIGITTE

Frontosphenoidal synostosis: a rare cause of unilateral anterior plagiocephaly
CHILD'S NERVOUS SYSTEM : CHNS : OFFICIAL JOURNAL OF THE INTERNATIONAL SOCIETY FOR PEDIATRIC NEUROSURGERY
2007 vol. 23(12) pp. 1431-1438
DE RIBAUPIERRE SANDRINE, CZORNY ALAIN, PITTET BRIGITTE, BERTRAND JACQUES, RILLIET BENEDICT

Aging is associated with an increased susceptibility to ischaemic necrosis due to microvascular perfusion failure but not a reduction in ischaemic tolerance
CLINICAL SCIENCE
2007 vol. 112 pp. 429-440
HARDER Y, AMON M, GEORGI M, SCHEUER C, SCHRAMM R, RUCKER M, PITTET B, ERNI D, MENGER MD

Selective blockade of endothelin-B receptor improves survival of critically perfused musculocutaneous flaps
LANGENBECKS ARCHIVES OF SURGERY
2007 vol. 392 pp. 331-338
WETTSTEIN R, MORSDORF P, BACHLE A, AMON M, PITTET B, MENGER MD, HARDER Y

Immunologic tolerance to skin allograft after graft-versus-host disease.
PLASTIC AND RECONSTRUCTIVE SURGERY
2007 vol. 119(3) pp. 40-44
ELIAS BG, MEUNIER DI, SQUIFFLET JP, MAROT L, WEYNAND B, VANWIJCK RR, BAYET B

Persistent ischemia impairs myofibroblast development in wound granulation tissue: a new model of delayed wound healing
WOUND REPAIR AND REGENERATION
2007 vol. 15(6) pp. 809-816
ALIZADEH NAVID, PEPPER MICHAEL SEAN, MODARRESSI ALI, ALFO KATIA, SCHLAUDRAFF KAI, MONTANDON DENYS, GABBIANI GIULIO, BOCHATON-PIALLAT MARIE-LUCE, PITTET BRIGITTE

Progressive tissue injury in burns is reduced by rNAPc2
BURNS
2006 vol. 32(8) pp. 957-963
MAHAJAN AL, TENORIO X, PEPPER MS, BAETENS D, MONTANDON D, SCHLAUDRAFF KU, PITTET B

The free serratus anterior flap and its cutaneous component for reconstruction of the face: A series of 27 cases
PLASTIC AND RECONSTRUCTIVE SURGERY
2006 vol. 117 pp. 1277-1288
PITTET-CUENOD B, MAHAJAN AL, ALIZADEH N, SCHLAUDRAFF KU, FASEL J, MONTANDON D

Early resuscitation with polymerized bovine hemoglobin reverses acidosis, but not peripheral tissue oxygenation, in a severe hamster shock model
SHOCK (AUGUSTA, GA.)
2006 vol. 26 pp. 496-503
WETTSTEIN R, TSAI AG, HARDER Y, ERNI D, INTAGLIETTA M

Adipose tissue is a regulated source of interleukin-10
CYTOKINE
2005 vol. 29 pp. 270-274
JUGE CRISTIANA, SOMM EMMANUEL, PERNIN CHOLLET AGNES, ALIZADEH NAVID, GIUSTI VITTORIO, DAYER JEAN-MICHEL, MEIER CHRISTOPH A

Infection in breast implants
THE LANCET INFECTIOUS DISEASES
2005 vol. 5 pp. 94-106
PITTET-CUENOD BRIGITTE, MONTANDON DENYS, PITTET DIDIER

[GESNOMA (Geneva Study Group on Noma): state-of-the-art medical research for humanitarian purposes].
ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE
2004 vol. 49(3) pp. 302-305
BARATTI-MAYER D, PITTET-CUENOD B, MONTANDON D

La chirurgie plastique humanitaire en question
ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE
2004 vol. 49 pp. 314-319
MONTANDON D, QUINODOZ P, PITTET-CUENOD B

Regulatory effects of interleukin (IL)-1, interferon-beta, and IL-4 on the production of IL-1 receptor antagonist by human adipose tissue
JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
2004 vol. 89 pp. 2652-2658
JUGE-AUBRY CE, SOMM E, CHICHEPORTICHE R, BURGER D, PERNIN A, PITTET-CUENOD B, QUINODOZ P, GIUSTI V, DAYER JM, MEIER CA

Active-site inactivated FVIIa decreases thrombosis and necrosis in a random skin flap model of acute ischemia
JOURNAL OF SURGICAL RESEARCH
2004 vol. 122 pp. 263-273
ALIZADEH N, PITTET-CUENOD BRIGITTE, TENORIO X, PIKE C, BAETENS D, SCHLAUDRAFF KU, MONTANDON D, EZBAN M, PEPPER MS

Simultaneous correction of major hypertelorism, frontal bone defect, nasal aplasia, and cleft of the upper lip (Tessier 0-14)
PLASTIC AND RECONSTRUCTIVE SURGERY
2004 vol. 113 pp. 299-303
PITTET B, JAQUINET A, RILLIET B, MONTANDON D

Noma: an "infectious" disease of unknown aetiology
THE LANCET INFECTIOUS DISEASES
2003 vol. 3 pp. 419-431
BARATTI-MAYER D, PITTET B, MONTANDON D, BOLIVAR I, BORNAND JE, HUGONNET S, JAQUINET A, SCHRENZEL J, PITTET D

Expansion labiale
ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE
2002 vol. 47 pp. 542-546
PITTET B, MONTANDON D

Reconstruction labiale dans les séquelles de noma
ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE
2002 vol. 47 pp. 520-535
MONTANDON D, PITTET B

Barium sulphate and soft-tissue radiology: allying the old and the new for the investigation of animal cutaneous microcirculation
BRITISH JOURNAL OF PLASTIC SURGERY
2002 vol. 55 pp. 664-667
QUINODOZ P, QUINODOZ M, NUSSBAUM JL, MONTANDON D, PITTET B

Skin flap-induced regression of granulation tissue correlates with reduced growth factor and increased metalloproteinase expression
JOURNAL OF PATHOLOGY
2002 vol. 197 pp. 117-127
DARBY IA, BISUCCI T, PITTET B, GABBIANI G, GARBIN S, DESMOULIERE A

La chirurgie esthétique après amaigrissement massif est une chirurgie reconstructive
MEDECINE ET HYGIENE : JOURNAL SUISSE D'INFORMATIONS MEDICALES
2002 vol. 60 pp. 1130-1133
QUINODOZ P, GUMENER R, PITTET B

[Correction of cervical contractures after burns with pedicled latissimus dorsi musculocutaneous flaps].
ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE
2001 vol. 46(3) pp. 235-242
PITTET-CUENOD B, CHERETAKIS A, MONTANDON D

Delivery of FGF-2 but not VEGF by encapsulated genetically engineered myoblasts improves survival and vascularization in a model of acute skin flap ischemia.
GENE THERAPY
2001 vol. 8 pp. 523-533
RINSCH C, QUINODOZ P, PITTET-CUENOD B, ALIZADEH N, BAETENS D, MONTANDON D, AEBISCHER P, PEPPER MS

Clinical experience in the treatment of noma sequelae.
THE JOURNAL OF CRANIOFACIAL SURGERY
2001 vol. 12(3) pp. 273-283
PITTET-CUENOD B, JAQUINET A, MONTANDON D

Hypoxia-inducible angiopoietin-2 expression is mimicked by iodonium compounds and occurs in the rat brain and skin in response to systemic hypoxia and tissue ischemia.
AMERICAN JOURNAL OF PATHOLOGY
2000 vol. 156(6) pp. 2077-2089
MANDRIOTA SJ, PYKE C, DI SANZA C, QUINODOZ P, PITTET-CUENOD B, PEPPER MS

[Falciform anemia and Plasmodium falciparum malaria: a threat to flap survival?].
ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE
1999 vol. 44(1) pp. 81-88
MARIETHOZ S, PITTET-CUENOD B, LOUTAN L, HUMBERT J, MONTANDON D

[Humanitarian plastic surgery. Personal experience and reflections].
ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE
1999 vol. 44(1) pp. 27-34
MONTANDON D, PITTET-CUENOD B

Craniofacial reconstruction of a giant cystlike temporal encephalocele.
THE JOURNAL OF CRANIOFACIAL SURGERY
1999 vol. 10(5) pp. 435-438
PITTET-CUENOD B, VIALOV S, RILLIET B, MONTANDON D

The effect of the delay phenomenon on the vascularity of rabbit abdominal cutaneous island flaps.
PLASTIC AND RECONSTRUCTIVE SURGERY
1997 vol. 99(1) pp. 183-193
CEDERNA PS, CHANG P, PITTET-CUENOD B, RAZABONI RM, CRAM AE

The effect of the delay phenomenon on the vascularity of rabbit rectus abdominis muscles.
PLASTIC AND RECONSTRUCTIVE SURGERY
1997 vol. 99(1) pp. 194-205
CEDERNA PS, CHANG P, PITTET-CUENOD B, RAZABONI RM, CRAM AE

Gigantic neurofibromatosis of the orbit.
THE JOURNAL OF CRANIOFACIAL SURGERY
1997 vol. ;8(6) pp. 497-500
PITTET-CUENOD B, GUMENER R, MONTANDON D

Immediate function of temporomandibular joint after total resection and reconstruction.
THE JOURNAL OF CRANIOFACIAL SURGERY
1997 vol. 8(5) pp. 383-390
RICHTER M, DULGUEROV P, PITTET-CUENOD B, BECKER M

The role of neovascularization in the survival of an arterialized venous flap
PLASTIC AND RECONSTRUCTIVE SURGERY
1996 vol. ;97(3) pp. 621-629
PITTET-CUENOD BRIGITTE, CHANG P, CEDERNA P, BLAIR WF, CRAM AE

The sandwich epicranial flaps.
PLASTIC AND RECONSTRUCTIVE SURGERY
1996 vol. 97(2) pp. 302-312
MONTANDON D, GUMENER R, PITTET B

Covering by a flap induces apoptosis of granulation tissue myofibroblasts and vascular cells.
WOUND REPAIR AND REGENERATION
1996 vol. 4(2) pp. 244-251
GARBIN S, PITTET B, MONTANDON D, GABBIANI G, DESMOULIČRE A

Effect of gamma-interferon on the clinical and biologic evolution of hypertrophic scars and Dupuytren's disease: an open pilot study
PLASTIC AND RECONSTRUCTIVE SURGERY
1994 vol. 93(6) pp. 1224-1235
PITTET-CUENOD BRIGITTE, RUBBIA BRANDT LAURA, DESMOULIERE A, SAPPINO PASCAL, ROGGERO P, GUERRET J, GRIMAUD JEROME, LACHER R, MONTANDON DENYS, GABBIANI GIULIO

Effect of gamma-interferon on the clinical and biologic evolution of hypertrophic scars and Dupuytren's disease: an open pilot study.
PLASTIC AND RECONSTRUCTIVE SURGERY
1994 vol. 93(6) pp. 1224-1235
PITTET-CUENOD B, RUBBIA-BRANDT L, DESMOULIERE A, SAPPINO AP, ROGGERO P, GUERRET S, GRIMAUD JA, LACHER R, MONTANDON D, GABBIANI G

Total anterior tenoarthrolysis to treat inveterate flexion contraction of the fingers: a series of 16 patients.
ANNALS OF PLASTIC SURGERY
1991 vol. 26(4) pp. 358-364
PITTET-CUENOD B, DELLA SANTA D, CHAMAY A

Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins.
LABORATORY INVESTIGATION
1989 vol. 60(2) pp. 275-285
SKALLI O, SCHÜRCH W, SEEMAYER T, LAGACÉ R, MONTANDON D, PITTET B, GABBIANI G

Research's domains




DATABASE-RESEARCH	GROUP OF CLINICAL PATIENT RESEARCH

Brigitte PITTET CUENOD	Head of group CV	Research subject	Members of the group


	Links about the group Pr. B. PITTET CUENOD HUG/ Dpt Chirurgie Serv Chir Plast et Reconst. Rue Gabrielle-Perret-Gentil 4 1211 Genève 14 Suisse Brigitte.Pittet-Cuenod@hcuge.ch Tel.: +41 22 372 80 12 Fax: +41 22 372 80 05 Comments Pages updated the 17.11.2015		Reseach's subject \| Group's publications \| Research's domains WOUND HEALING, MICROCIRCULATION AND ISCHEMIA, FLAP SURGERY WOUND HEALING - Hypertrophic scars and keloids. Histological and clinical follow-up. Clinical trial with g interferon, local radiotherapy and chemotherapy associated with surgical excision (Prof Gabbiani). - Wound healing physiology and the role of apoptosis in the remodelling phase of the granulation tissue to a scar has been studied on a wound rat model. Induction of myofibroblast apoptosis by wound coverage with non-vascularized (skin grafts) or vascularized tissue (skin, muscle or fat flaps) (Prof Gabbiani). - Wound healing and ischemia: description of a new animal model of ischemic wounds in rat. Correlation between wound healing delay, decrease in wound contraction and a significant delay in myofibroblast appearance in the wound as well as a decrease in the absolute number of myofibroblasts (Prof Gabbiani, Prof Pepper). MICROCIRCULATION AND ISCHEMIA 1)SURVIVAL OF ISCHEMIC TISSUE - Experimental study on physiology, histology of tissue ischemia and the onset of irreversible necrosis on an ischemic skin flap model in rats. Expression of endogenous VEGF and its tyrosine kinase receptors has been measured in the flap by immunohistochemistry to demonstrate the presence of angiogenesis. VEGF and bFGF were applied under the flap to stimulate in situ angiogenesis using an ex vivo gene therapy approach with transfected cells placed under the flap in semi-permeable capsules to increase flap survival (Prof Pepper, Prof Aebischer). - Dynamic study of cutaneous blood flow under ischemic condition has been carried out using laser-doppler analysis and microangiography. Inhibition of microvascular thrombosis by parenteral administration of various coagulation inhibitors (inactivated factor VII, rNAPc2) to decrease tissue necrosis under ischemic condition has been successfully tested (Prof Pepper). The efficacy of intermittent hypoxia training (IHT) as a hypoxic preconditioning procedure has been tested. IHT consisted of 20 daily sessions of alternative exposure of hypoxic gas mixture and ambient air. A significant decrease of PO2 during IHT sessions, with a tendency to disappear after 4 weeks of IHT, has been demonstrated. Preconditioning with IHT led to a significant increase of overall flap survival. Analysis of mRNA-expression of vascular endothelial growth factor (VEGF) and its receptors in skin, as well as in the flap, after a 4 week course of IHT to compare rats preconditioned with IHT versus a control group, did not reveal a significant difference. 2) MECHANISMS OF SECONDARY AGGRAVATION OF BURN LESIONS Development of an animal model of burn lesion in rats. Analysis of the microcirculation around the lesion by laser-doppler and microangiography to demonstrate the secondary aggravation of the burn lesion. Administration of coagulation inhibitor (rNAPc2) to stop the mechanism of secondary burn extension(Prof Pepper) FLAP SURGERYARTERIALIZED-VENOUS FLAPS Experimental research on new types of flaps: the arterialized-venous flap. Development of the animal model (rabbit ear), study of the mechanism of survival and the role of neovascularization in this survival. Anatomical classification of the various types of arterialized-venous flap; study of the maximal surface of flap survival based on the anatomy of the vascular venous tree within the flap. 2) ANATOMICAL STUDY Description of a new flap ; the serratus anterior musculo-cutaneous . Based on anatomical dissection, description of the cutaneous perforator vessels reaching the skin paddle (Prof Fasel ). Group's publications Locating perforator vessels by dynamic infrared imaging and flow Doppler with no thermal cold challenge. ANNALS OF PLASTIC SURGERY 2011 vol. 67(2) pp. 143-146 TENORIO X, MAHAJAN AL, ELIAS B, VAN RIEMPST JS, WETTSTEIN R, HARDER Y, PITTET-CUENOD B A facial inflammatory myofibroblastic tumour in a 6-year-old girl: plastic surgery lessons from a rare case. JOURNAL OF CRANIO-MAXILLO-FACIAL SURGERY : OFFICIAL PUBLICATION OF THE EUROPEAN ASSOCIATION FOR CRANIO-MAXILLO-FACIAL SURGERY 2011 vol. 39(2) pp. 141-144 MODARRESSI A, PIETRAMAGGIORI G, LACROIX S, RILLIET B, PITTET-CUENOD B Superior pedicle breast reduction for hypertrophy with massive ptosis. JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY 2011 vol. 64(4) pp. 500-507 WETTSTEIN R, CHRISTOFIDES E, PITTET-CUENOD B, PSARAS G, HARDER Y Nonactivated versus thrombin-activated platelets on wound healing and fibroblast-to-myofibroblast differentiation in vivo and in vitro. PLASTIC AND RECONSTRUCTIVE SURGERY 2011 vol. 129(1) pp. 46-54 SCHERER SS, TOBALEM M, VIGATO E, HEIT Y, MODARRESSI A, HINZ B, PITTET-CUENOD B, PIETRAMAGGIORI G Frostbite complicating therapeutic surface cooling after heat stroke. INTENSIVE CARE MEDICINE 2010 vol. 36(9) pp. 1614-1615 TOBALEM M, MODARRESSI A, ELIAS B, HARDER Y, PITTET-CUENOD B Hypoxia impairs skin myofibroblast differentiation and function. JOURNAL OF INVESTIGATIVE DERMATOLOGY 2010 vol. 130(12) pp. 2818-2827 MODARRESSI A, PIETRAMOGGIORI G, GODBOUT C, VIGATO E, PITTET B, HINZ B [Orbitotemporal facial involvement in type 1 neurofibromatosis (NF1)]. NEURO-CHIRURGIE 2010 vol. 56(2-3) pp. 257-270 RILLIET B, PITTET-CUENOD B, MONTANDON D, NARATA AP, DE RIBEAUPIERRE S, SCHILS F, BOSCHERINI D, DI ROCCO C, DUCREY N Body taping for contour surgery. AESTHETIC PLASTIC SURGERY 2009 vol. 33(3) pp. 324-326 KALBERMATTEN DF, WETTSTEIN R, ERBA P, RIEGER UM, PIERER G, RAFFOUL W [About 10 cases of Launois-Bensaude disease]. ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE 2009 vol. 54(2) pp. 135-145 PAUCHOT J, GOLAY A, GUMENER R, MONTANDON D, PITTET-CUENOD B Osseous alterations at the interface of hydrogel expanders and underlying bone. JOURNAL OF CRANIO-MAXILLO-FACIAL SURGERY : OFFICIAL PUBLICATION OF THE EUROPEAN ASSOCIATION FOR CRANIO-MAXILLO-FACIAL SURGERY 2009 vol. 37(5) pp. 258-262 STUEHMER C, RUCKER M, SCHUMANN P, BORMANN KH, HARDER Y, SINIKOVIC B, GELLRICH NC Improved cutaneous healing in diabetic mice exposed to healthy peripheral circulation. JOURNAL OF INVESTIGATIVE DERMATOLOGY 2009 vol. 129(9) pp. 2265-2274 PIETRAMOGGIORI G, SCHERER SS, ALPEROVICH M, CHEN B, ORGILL DP, WAGERS AF Early detection of flap failure using a new thermographic device. JOURNAL OF SURGICAL RESEARCH 2009 vol. 151(1) pp. 15-21 TENORIO X, MAHAJAN AL, WETTSTEIN R, HARDER Y, PAWLOVSKI M, PITTET B Erythropoietin reduces necrosis in critically ischemic myocutaneous tissue by protecting nutritive perfusion in a dose-dependent manner. SURGERY 2009 vol. 145(4) pp. 372-383 HARDER Y, AMON M, SCHRAMM R, CONTALDO C, METZKOW E, MATZEN A, RUCKER M, VOLLMAR B, MENGER MD Hemiface rhytidectomy. AESTHETIC PLASTIC SURGERY 2008 vol. 32(2) pp. 227-233 KALBERMATTEN DF, ERBA P, WETTSTEIN R, PIERER G Erythropoietin protects critically perfused flap tissue. ANNALS OF SURGERY 2008 vol. 248(6) pp. 919-929 REZAEIAN F, WETTSTEIN R, AMON M, SCHEUER C, SCHRAMM R, MENGER MD, PITTET-CUENOD B, HARDER Y Microvascular experimental evidence on the relative significance of restoring oxygen carrying capacity vs. blood viscosity in shock resuscitation. BIOCHIMICA ET BIOPHYSICA ACTA : INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND BIOPHYSICS. 2008 vol. 1784(10) pp. 1421-1427 SALAZAR VÁZQUEZ BY, WETTSTEIN R, CABRALES P, TSAI AG, INTAGLIETTA M Monocyte chemoattractant protein-1 secreted by adipose tissue induces direct lipid accumulation in hepatocytes. HEPATOLOGY 2008 vol. 48(3) pp. 799-807 CLEMENT S, JUGE-AUBRY CRISTIANA, SGROI A, CONZELMAN S, PAZIENZA VALERIO, PITTET-CUENOD B, MEIER CA, NEGRO F Hypoxic preconditioning increases skin oxygenation and viability but does not alter VEGF expression or vascular density. HIGH ALTITUDE MEDICINE & BIOLOGY 2008 vol. 9(1) pp. 76-88 SCHLAUDRAFF KU, PEPPER MS, TKATCHOUK EN, EHRENBURG I, ALIZADEH N, MONTANDON D, PITTET B Ischemia-induced up-regulation of heme oxygenase-1 protects from apoptotic cell death and tissue necrosis. JOURNAL OF SURGICAL RESEARCH 2008 vol. 150(2) pp. 293-303 HARDER Y, AMON M, SCHRAMM R, RUCKER M, SCHEUER C, PITTET B, ERNI D, MENGER MD Mixed arterio-venous insufficiency in random skin flaps in the rat: is the application of medicinal leeches beneficial? JOURNAL OF SURGICAL RESEARCH 2008 vol. 150(1) pp. 85-91 SCHLAUDRAFF KU, BEZZOLA T, MONTANDON D, PEPPER MS, PITTET B Optimizing the arterialized venous flap. PLASTIC AND RECONSTRUCTIVE SURGERY 2008 vol. 122(6) pp. 1681-1689 PITTET-CUENOD B, QUINODOZ P, ALIZADEH N, SCHLAUDRAFF KU, MAHAJAN AL An old dream revitalised: preconditioning strategies to protect surgical flaps from critical ischaemia and ischaemia-reperfusion injury. THE JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGICAL NURSING : OFFICIAL ORGAN OF THE AMERICAN SOCIETY OF PLASTIC AND RECONSTRUCTIVE SURGICAL NURSES 2008 vol. 61(5) pp. 503-511 HARDER Y AND AL. Review of 197 consecutive free flap reconstructions in the lower extremity. THE JOURNAL OF PLASTIC AND RECONSTRUCTIVE SURGICAL NURSING : OFFICIAL ORGAN OF THE AMERICAN SOCIETY OF PLASTIC AND RECONSTRUCTIVE SURGICAL NURSES 2008 vol. 61(7) pp. 772-776 WETTSTEIN R, SCHURCH R, BANIC A, ERNI D, HARDER Y [Letters to the editor]. ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE 2007 vol. 52(6) pp. 628-629 PITTET-CUENOD B Mastectomie avec préservation cutanée et reconstruction immédiate BULLETIN DU CANCER : JOURNAL DE LA SOCIETE FRANCAISE DU CANCER 2007 vol. 94(9) pp. 833-840 VLASTOS GEORGES, ELIAS B, MEYER OLNA, HARDER YVES, SCHÄFER PIERRE, PITTET BRIGITTE Frontosphenoidal synostosis: a rare cause of unilateral anterior plagiocephaly CHILD'S NERVOUS SYSTEM : CHNS : OFFICIAL JOURNAL OF THE INTERNATIONAL SOCIETY FOR PEDIATRIC NEUROSURGERY 2007 vol. 23(12) pp. 1431-1438 DE RIBAUPIERRE SANDRINE, CZORNY ALAIN, PITTET BRIGITTE, BERTRAND JACQUES, RILLIET BENEDICT Aging is associated with an increased susceptibility to ischaemic necrosis due to microvascular perfusion failure but not a reduction in ischaemic tolerance CLINICAL SCIENCE 2007 vol. 112 pp. 429-440 HARDER Y, AMON M, GEORGI M, SCHEUER C, SCHRAMM R, RUCKER M, PITTET B, ERNI D, MENGER MD Selective blockade of endothelin-B receptor improves survival of critically perfused musculocutaneous flaps LANGENBECKS ARCHIVES OF SURGERY 2007 vol. 392 pp. 331-338 WETTSTEIN R, MORSDORF P, BACHLE A, AMON M, PITTET B, MENGER MD, HARDER Y Immunologic tolerance to skin allograft after graft-versus-host disease. PLASTIC AND RECONSTRUCTIVE SURGERY 2007 vol. 119(3) pp. 40-44 ELIAS BG, MEUNIER DI, SQUIFFLET JP, MAROT L, WEYNAND B, VANWIJCK RR, BAYET B Persistent ischemia impairs myofibroblast development in wound granulation tissue: a new model of delayed wound healing WOUND REPAIR AND REGENERATION 2007 vol. 15(6) pp. 809-816 ALIZADEH NAVID, PEPPER MICHAEL SEAN, MODARRESSI ALI, ALFO KATIA, SCHLAUDRAFF KAI, MONTANDON DENYS, GABBIANI GIULIO, BOCHATON-PIALLAT MARIE-LUCE, PITTET BRIGITTE Progressive tissue injury in burns is reduced by rNAPc2 BURNS 2006 vol. 32(8) pp. 957-963 MAHAJAN AL, TENORIO X, PEPPER MS, BAETENS D, MONTANDON D, SCHLAUDRAFF KU, PITTET B The free serratus anterior flap and its cutaneous component for reconstruction of the face: A series of 27 cases PLASTIC AND RECONSTRUCTIVE SURGERY 2006 vol. 117 pp. 1277-1288 PITTET-CUENOD B, MAHAJAN AL, ALIZADEH N, SCHLAUDRAFF KU, FASEL J, MONTANDON D Early resuscitation with polymerized bovine hemoglobin reverses acidosis, but not peripheral tissue oxygenation, in a severe hamster shock model SHOCK (AUGUSTA, GA.) 2006 vol. 26 pp. 496-503 WETTSTEIN R, TSAI AG, HARDER Y, ERNI D, INTAGLIETTA M Adipose tissue is a regulated source of interleukin-10 CYTOKINE 2005 vol. 29 pp. 270-274 JUGE CRISTIANA, SOMM EMMANUEL, PERNIN CHOLLET AGNES, ALIZADEH NAVID, GIUSTI VITTORIO, DAYER JEAN-MICHEL, MEIER CHRISTOPH A Infection in breast implants THE LANCET INFECTIOUS DISEASES 2005 vol. 5 pp. 94-106 PITTET-CUENOD BRIGITTE, MONTANDON DENYS, PITTET DIDIER [GESNOMA (Geneva Study Group on Noma): state-of-the-art medical research for humanitarian purposes]. ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE 2004 vol. 49(3) pp. 302-305 BARATTI-MAYER D, PITTET-CUENOD B, MONTANDON D La chirurgie plastique humanitaire en question ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE 2004 vol. 49 pp. 314-319 MONTANDON D, QUINODOZ P, PITTET-CUENOD B Regulatory effects of interleukin (IL)-1, interferon-beta, and IL-4 on the production of IL-1 receptor antagonist by human adipose tissue JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM 2004 vol. 89 pp. 2652-2658 JUGE-AUBRY CE, SOMM E, CHICHEPORTICHE R, BURGER D, PERNIN A, PITTET-CUENOD B, QUINODOZ P, GIUSTI V, DAYER JM, MEIER CA Active-site inactivated FVIIa decreases thrombosis and necrosis in a random skin flap model of acute ischemia JOURNAL OF SURGICAL RESEARCH 2004 vol. 122 pp. 263-273 ALIZADEH N, PITTET-CUENOD BRIGITTE, TENORIO X, PIKE C, BAETENS D, SCHLAUDRAFF KU, MONTANDON D, EZBAN M, PEPPER MS Simultaneous correction of major hypertelorism, frontal bone defect, nasal aplasia, and cleft of the upper lip (Tessier 0-14) PLASTIC AND RECONSTRUCTIVE SURGERY 2004 vol. 113 pp. 299-303 PITTET B, JAQUINET A, RILLIET B, MONTANDON D Noma: an "infectious" disease of unknown aetiology THE LANCET INFECTIOUS DISEASES 2003 vol. 3 pp. 419-431 BARATTI-MAYER D, PITTET B, MONTANDON D, BOLIVAR I, BORNAND JE, HUGONNET S, JAQUINET A, SCHRENZEL J, PITTET D Expansion labiale ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE 2002 vol. 47 pp. 542-546 PITTET B, MONTANDON D Reconstruction labiale dans les séquelles de noma ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE 2002 vol. 47 pp. 520-535 MONTANDON D, PITTET B Barium sulphate and soft-tissue radiology: allying the old and the new for the investigation of animal cutaneous microcirculation BRITISH JOURNAL OF PLASTIC SURGERY 2002 vol. 55 pp. 664-667 QUINODOZ P, QUINODOZ M, NUSSBAUM JL, MONTANDON D, PITTET B Skin flap-induced regression of granulation tissue correlates with reduced growth factor and increased metalloproteinase expression JOURNAL OF PATHOLOGY 2002 vol. 197 pp. 117-127 DARBY IA, BISUCCI T, PITTET B, GABBIANI G, GARBIN S, DESMOULIERE A La chirurgie esthétique après amaigrissement massif est une chirurgie reconstructive MEDECINE ET HYGIENE : JOURNAL SUISSE D'INFORMATIONS MEDICALES 2002 vol. 60 pp. 1130-1133 QUINODOZ P, GUMENER R, PITTET B [Correction of cervical contractures after burns with pedicled latissimus dorsi musculocutaneous flaps]. ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE 2001 vol. 46(3) pp. 235-242 PITTET-CUENOD B, CHERETAKIS A, MONTANDON D Delivery of FGF-2 but not VEGF by encapsulated genetically engineered myoblasts improves survival and vascularization in a model of acute skin flap ischemia. GENE THERAPY 2001 vol. 8 pp. 523-533 RINSCH C, QUINODOZ P, PITTET-CUENOD B, ALIZADEH N, BAETENS D, MONTANDON D, AEBISCHER P, PEPPER MS Clinical experience in the treatment of noma sequelae. THE JOURNAL OF CRANIOFACIAL SURGERY 2001 vol. 12(3) pp. 273-283 PITTET-CUENOD B, JAQUINET A, MONTANDON D Hypoxia-inducible angiopoietin-2 expression is mimicked by iodonium compounds and occurs in the rat brain and skin in response to systemic hypoxia and tissue ischemia. AMERICAN JOURNAL OF PATHOLOGY 2000 vol. 156(6) pp. 2077-2089 MANDRIOTA SJ, PYKE C, DI SANZA C, QUINODOZ P, PITTET-CUENOD B, PEPPER MS [Falciform anemia and Plasmodium falciparum malaria: a threat to flap survival?]. ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE 1999 vol. 44(1) pp. 81-88 MARIETHOZ S, PITTET-CUENOD B, LOUTAN L, HUMBERT J, MONTANDON D [Humanitarian plastic surgery. Personal experience and reflections]. ANNALES DE CHIRURGIE PLASTIQUE ET ESTHETIQUE 1999 vol. 44(1) pp. 27-34 MONTANDON D, PITTET-CUENOD B Craniofacial reconstruction of a giant cystlike temporal encephalocele. THE JOURNAL OF CRANIOFACIAL SURGERY 1999 vol. 10(5) pp. 435-438 PITTET-CUENOD B, VIALOV S, RILLIET B, MONTANDON D The effect of the delay phenomenon on the vascularity of rabbit abdominal cutaneous island flaps. PLASTIC AND RECONSTRUCTIVE SURGERY 1997 vol. 99(1) pp. 183-193 CEDERNA PS, CHANG P, PITTET-CUENOD B, RAZABONI RM, CRAM AE The effect of the delay phenomenon on the vascularity of rabbit rectus abdominis muscles. PLASTIC AND RECONSTRUCTIVE SURGERY 1997 vol. 99(1) pp. 194-205 CEDERNA PS, CHANG P, PITTET-CUENOD B, RAZABONI RM, CRAM AE Gigantic neurofibromatosis of the orbit. THE JOURNAL OF CRANIOFACIAL SURGERY 1997 vol. ;8(6) pp. 497-500 PITTET-CUENOD B, GUMENER R, MONTANDON D Immediate function of temporomandibular joint after total resection and reconstruction. THE JOURNAL OF CRANIOFACIAL SURGERY 1997 vol. 8(5) pp. 383-390 RICHTER M, DULGUEROV P, PITTET-CUENOD B, BECKER M The role of neovascularization in the survival of an arterialized venous flap PLASTIC AND RECONSTRUCTIVE SURGERY 1996 vol. ;97(3) pp. 621-629 PITTET-CUENOD BRIGITTE, CHANG P, CEDERNA P, BLAIR WF, CRAM AE The sandwich epicranial flaps. PLASTIC AND RECONSTRUCTIVE SURGERY 1996 vol. 97(2) pp. 302-312 MONTANDON D, GUMENER R, PITTET B Covering by a flap induces apoptosis of granulation tissue myofibroblasts and vascular cells. WOUND REPAIR AND REGENERATION 1996 vol. 4(2) pp. 244-251 GARBIN S, PITTET B, MONTANDON D, GABBIANI G, DESMOULIČRE A Effect of gamma-interferon on the clinical and biologic evolution of hypertrophic scars and Dupuytren's disease: an open pilot study PLASTIC AND RECONSTRUCTIVE SURGERY 1994 vol. 93(6) pp. 1224-1235 PITTET-CUENOD BRIGITTE, RUBBIA BRANDT LAURA, DESMOULIERE A, SAPPINO PASCAL, ROGGERO P, GUERRET J, GRIMAUD JEROME, LACHER R, MONTANDON DENYS, GABBIANI GIULIO Effect of gamma-interferon on the clinical and biologic evolution of hypertrophic scars and Dupuytren's disease: an open pilot study. PLASTIC AND RECONSTRUCTIVE SURGERY 1994 vol. 93(6) pp. 1224-1235 PITTET-CUENOD B, RUBBIA-BRANDT L, DESMOULIERE A, SAPPINO AP, ROGGERO P, GUERRET S, GRIMAUD JA, LACHER R, MONTANDON D, GABBIANI G Total anterior tenoarthrolysis to treat inveterate flexion contraction of the fingers: a series of 16 patients. ANNALS OF PLASTIC SURGERY 1991 vol. 26(4) pp. 358-364 PITTET-CUENOD B, DELLA SANTA D, CHAMAY A Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins. LABORATORY INVESTIGATION 1989 vol. 60(2) pp. 275-285 SKALLI O, SCHÜRCH W, SEEMAYER T, LAGACÉ R, MONTANDON D, PITTET B, GABBIANI G Research's domains