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Circadian Endocrinology.

Circadian oscillation of biological processes has been described in most of the light-sensitive organisms on earth. It reflects the existence of underlying intrinsic biological clocks with near 24 hour oscillation periods. It is well established that circadian clocks play a crucial role in the regulation of key metabolic processes. Moreover, there is an emerging evidence for connection between metabolic pathologies and the circadian clockwork. The long-term goal of our laboratory is to identify the molecular basis of circadian rhythmicity in rodent and human peripheral tissues in physiological, and in obesity/type 2 diabetes conditions. We have setup the experimental system for long-term recording of circadian reporter oscillations in human primary cultured cells from different tissue types at population and single cell levels. Using this powerful approach, we scrutinize the role of the oscillators present in a- and ß- cells in pancreatic islet function, and the impact of glucose metabolism on a- and ß- cell oscillators, in mouse and human models. Also, human skeletal muscle clock molecular makeup and its roles in regulating myokine secretion and insulin resistance development have been tackled. The prevalence of obesity and diabetes in modern society is taking on enormous proportions. It is therefore of major importance to identify the molecular basis of circadian rhythmicity in rodent and human peripheral tissues in physiological conditions, and upon obesity/T2D. In addition, we are interested in the connection between circadian clock and cancer, and in particular the thyroid cancer. We explore the molecular makeup of human thyroid clocks in physiology and upon thyroid malignancies, in an attempt to bring new insights into the role of circadian clocks in thyroid cancer, and to apply these changes for thyroid malignancies diagnostics in clinics.

Group's publications

Autonomous and self-sustained circadian oscillators displayed in human islet cells.
DIABETOLOGIA
2013 vol. 56(3) pp. 497-507
PULIMENO P AND AL.

Circadian clock characteristics are altered in human thyroid malignant nodules.
JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
2013 vol. 98(11) pp. 4446-4456
MANNIC T, MEYER P, TRIPONEZ F, PUSZTASZERI M, LE MARTELOT G, MARIANI O, SCHMITTER D, SAGE D, PHILIPPE J, DIBNER C

Glucagon gene expression in the endocrine pancreas: the role of the transcription factor Pax6 in ż-cell differentiation, glucagon biosynthesis and secretion.
DIABETES, OBESITY AND METABOLISM
2011 vol. 13 pp. 31-38
GOSMAIN Y, CHEYSSAC C, HEDDAD MASSON M, DIBNER C, PHILIPPE J

The mammalian circadian timing system: organization and coordination of central and peripheral clocks.
ANNUAL REVIEW OF PHYSIOLOGY
2010 vol. 72 pp. 517-549
DIBNER C, SCHIBLER U, ALBRECHT U

A software solution for recording circadian oscillator features in time-lapse live cell microscopy.
CELL DIVISION
2010 vol. 17 pp. 17-17
SAGE D, UNSER M, SALMON P, DIBNER C

Xenopus Meis3 protein lies at a nexus downstream to Zic1 and Pax3 proteins, regulating multiple cell-fates during early nervous system development.
DEVELOPMENTAL BIOLOGY
2010 vol. 338(1) pp. 50-62
GUTKOVICH YE, OFIR R, ELKOUBY Y, DIBNER C, GEFEN A, ELIAS S, FRANCK D

The circadian clock starts ticking at a developmentally early stage.
JOURNAL OF BIOLOGICAL RHYTHMS
2010 vol. 25(6) pp. 442-449
KOWALSKA E, MORIGGI E, BAUER C, DIBNER C, BROWN SA

On the robustness of mammalian circadian oscillators.
CELL CYCLE
2009 vol. 8(5) pp. 681-682
DIBNER C

Circadian gene expression is resilient to large fluctuations in overall transcription rates
EMBO JOURNAL
2009 vol. 28(2) pp. 123-134
DIBNER C, SAGE D, UNSER M, BAUER C, SCHIBLER U, D'EYSMOND T, NAEF F

SIRT1 regulates circadian clock gene expression through PER2 deacetylation.
CELL
2008 vol. 134(2) pp. 317-328
ASHER G, GATFIELD D, STRATMANN M, REINKE H, DIBNER C, KREPPEL F, MOSTOSLAVSKY R, ALT FW, SCHIBLER U

Differential display of DNA-binding proteins reveals heat-shock factor 1 as a circadian transcription factor.
GENES AND DEVELOPMENT
2008 vol. 22(3) pp. 331-345
REINKE H, SAINI C, FLEURY-OLELA F, DIBNER C, BENJAMIN IJ, SCHIBLER U

Research's domains




DATABASE-RESEARCH	GROUP OF CLINICAL PATIENT RESEARCH

Doctor Dibner Charna	Head of group CV	Research subject	Members of the group


	Links about the group Docteure Dibner CHARNA HUG/Spécialités de Médecine Serv. Endo.Diabet.Nutrit. Rue Gabrielle-Perret-Gentil 4 1211 Genève 14 Suisse Charna.Dibner@hcuge.ch Tel.: +41 22 372 93 18 Fax: +41 22 372 93 26 Group's web site / department Comments Pages updated the 17.11.2015		Reseach's subject \| Group's publications \| Research's domains Circadian Endocrinology. Circadian oscillation of biological processes has been described in most of the light-sensitive organisms on earth. It reflects the existence of underlying intrinsic biological clocks with near 24 hour oscillation periods. It is well established that circadian clocks play a crucial role in the regulation of key metabolic processes. Moreover, there is an emerging evidence for connection between metabolic pathologies and the circadian clockwork. The long-term goal of our laboratory is to identify the molecular basis of circadian rhythmicity in rodent and human peripheral tissues in physiological, and in obesity/type 2 diabetes conditions. We have setup the experimental system for long-term recording of circadian reporter oscillations in human primary cultured cells from different tissue types at population and single cell levels. Using this powerful approach, we scrutinize the role of the oscillators present in a- and ß- cells in pancreatic islet function, and the impact of glucose metabolism on a- and ß- cell oscillators, in mouse and human models. Also, human skeletal muscle clock molecular makeup and its roles in regulating myokine secretion and insulin resistance development have been tackled. The prevalence of obesity and diabetes in modern society is taking on enormous proportions. It is therefore of major importance to identify the molecular basis of circadian rhythmicity in rodent and human peripheral tissues in physiological conditions, and upon obesity/T2D. In addition, we are interested in the connection between circadian clock and cancer, and in particular the thyroid cancer. We explore the molecular makeup of human thyroid clocks in physiology and upon thyroid malignancies, in an attempt to bring new insights into the role of circadian clocks in thyroid cancer, and to apply these changes for thyroid malignancies diagnostics in clinics. Group's publications Autonomous and self-sustained circadian oscillators displayed in human islet cells. DIABETOLOGIA 2013 vol. 56(3) pp. 497-507 PULIMENO P AND AL. Circadian clock characteristics are altered in human thyroid malignant nodules. JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM 2013 vol. 98(11) pp. 4446-4456 MANNIC T, MEYER P, TRIPONEZ F, PUSZTASZERI M, LE MARTELOT G, MARIANI O, SCHMITTER D, SAGE D, PHILIPPE J, DIBNER C Glucagon gene expression in the endocrine pancreas: the role of the transcription factor Pax6 in ż-cell differentiation, glucagon biosynthesis and secretion. DIABETES, OBESITY AND METABOLISM 2011 vol. 13 pp. 31-38 GOSMAIN Y, CHEYSSAC C, HEDDAD MASSON M, DIBNER C, PHILIPPE J The mammalian circadian timing system: organization and coordination of central and peripheral clocks. ANNUAL REVIEW OF PHYSIOLOGY 2010 vol. 72 pp. 517-549 DIBNER C, SCHIBLER U, ALBRECHT U A software solution for recording circadian oscillator features in time-lapse live cell microscopy. CELL DIVISION 2010 vol. 17 pp. 17-17 SAGE D, UNSER M, SALMON P, DIBNER C Xenopus Meis3 protein lies at a nexus downstream to Zic1 and Pax3 proteins, regulating multiple cell-fates during early nervous system development. DEVELOPMENTAL BIOLOGY 2010 vol. 338(1) pp. 50-62 GUTKOVICH YE, OFIR R, ELKOUBY Y, DIBNER C, GEFEN A, ELIAS S, FRANCK D The circadian clock starts ticking at a developmentally early stage. JOURNAL OF BIOLOGICAL RHYTHMS 2010 vol. 25(6) pp. 442-449 KOWALSKA E, MORIGGI E, BAUER C, DIBNER C, BROWN SA On the robustness of mammalian circadian oscillators. CELL CYCLE 2009 vol. 8(5) pp. 681-682 DIBNER C Circadian gene expression is resilient to large fluctuations in overall transcription rates EMBO JOURNAL 2009 vol. 28(2) pp. 123-134 DIBNER C, SAGE D, UNSER M, BAUER C, SCHIBLER U, D'EYSMOND T, NAEF F SIRT1 regulates circadian clock gene expression through PER2 deacetylation. CELL 2008 vol. 134(2) pp. 317-328 ASHER G, GATFIELD D, STRATMANN M, REINKE H, DIBNER C, KREPPEL F, MOSTOSLAVSKY R, ALT FW, SCHIBLER U Differential display of DNA-binding proteins reveals heat-shock factor 1 as a circadian transcription factor. GENES AND DEVELOPMENT 2008 vol. 22(3) pp. 331-345 REINKE H, SAINI C, FLEURY-OLELA F, DIBNER C, BENJAMIN IJ, SCHIBLER U Research's domains