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BASE DE DONNÉES-RECHERCHE | |
GROUPE DE RECHERCHE CLINIQUE ORIENTEE BIOLOGIE | ||||
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Francesco NEGRO | CV du Chef de Groupe | Sujet de Recherche | Composition du Groupe | |||
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Coordonnées du groupe
Dr. F. NEGRO CMU / Dpt DEMED Laboratoire Gastro-Entérologie 1 rue Michel-Servet 1205 Genève Suisse negro-francesco@diogenes.hcuge.ch Tél.: +41 22 379 58 00 / 95069 Fax: +41 22 372 93 66 Commentaires Pages générées le 16.05.2012 |
Sujet de recherche
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Publications du groupe
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Domaines de recherche
Etudes et traitement de l'hépatite C et B
Conduite d'études cliniques de traitement:
1. Traitement par interféron alpha-2a pégylé e ribavirine de patients atteints d'une hépatite C recourant après greffe de foie (étude SASL 17), avec étude de la cinétique de l'ARN viral C circulant (Investigateur principal) 2. Traitement par interféron alpha-2b pégylé en monothérapie de patients atteints d'une hépatite virale C aiguë (étude SASL 18), avec analyse de la cinétique de l'ARN viral C (sérum, PBMC) et de certains paramètres immunologiques (investigateur principal) 3. Vaccinothérapie avec protéine de l'enveloppe 2 du virus de l'hépatite C de patients avec hépatite C chronique n'ayant pas répondu au traitement antiviral standard (étude Innogenetics #916) (Co-investigateur d'une étude multicentrique européenne dirigée par le Dr. Hulstaert, Innogenetics, Ghent, Belgique) 4. Analyse de la cinétique de l'ARN viral C dans les cellules mononuclées du sang périphérique pendant traitement combiné avec interféron alpha-2a pégylé e ribavirine de patients atteints d'une hépatite C chronique, et corrélation avec d'autres paramètres virologiques et immunologiques (Etude DITTO-HCV) (Co-investigateur d'une étude multicentrique européenne dirigée par le Dr. A. Neumann, Bar-Ilan University, Israel) Organisation et gestion d'une Etude de Cohorte de patients atteints d'une hépatite C chronique à l'échelon suisse (Président) Participation au Réseau d'Excellence "Virgil", financé par la Commission Européenne, dont le but est celui de caractériser la résistance des virus de l'hépatite B et C et de la grippe aux différents antiviraux. La contribution au réseau par laboratoire dirigé par le Dr Negro consiste à: 1. participer au développement d'un système de réplication in vitro du virus de l'hépatite C subgénomique (principe du "replicon") de génotype 3a; 2. caractériser les patients atteints d'une hépatite C aiguë ou chronique et non-répondeurs aux traitement antiviraux courants par le moyen d'une méthode de quantification de l'ARN viral C en temps réel (TaqMan) dans les PBMC Etude de la pathogenèse des lésions hépatiques et extrahépatiques dans l'hépatite virale C chronique, chez les individus immunocompétents et immunodéprimés. La recherche a comme buts: ˇ l'étude de la pathogenèse de la stéatose hépatique, avec analyse des déterminants viraux (taux de multiplication virale C, génotype viral) et de l'hôte (peroxidation lipidique, résistance à l'insuline, système de la leptine) ˇ l'établissement d'un modèle de stéatose in vitro par transfection avec différents gènes du VHC ˇ l'étude du rapport entre hétérogénéité de séquence virale C et manifestations extra-hépatiques Publications du groupe Hepatitis delta virus inhibits alpha interferon signaling. HEPATOLOGY 2009 vol. 49(2) pp. 398-406 PUGNALE P, PAZIENZA V, GUILLOUX K, NEGRO F Chronic hepatitis E in the immunosuppressed: a new source of trouble? JOURNAL OF HEPATOLOGY 2009 vol. 50(2) pp. 435-437 BIHL F, NEGRO F Correction of insulin resistance in chronic hepatitis C patients not responding to the standard of care: more questions than answers. JOURNAL OF HEPATOLOGY 2009 vol. 50(6) pp. 1271-1272 NEGRO F Genotype 3 is associated with accelerated fibrosis progression in chronic hepatitis C. JOURNAL OF HEPATOLOGY 2009 vol. 51(4) pp. 655-666 BOCHUD PY AND AL. Impact of obesity steatosis and insulin resistance on progression and response to therapy of hepatitis C JOURNAL OF VIRAL HEPATITIS 2009 vol. 16(9) pp. 681-688 NEGRO F, CLEMENT S Little evidence that hepatitis C virus leads to a higher risk of mortality in the absence of cirrhosis and excess alcohol intake: the Swiss Hepatitis C Cohort Study. JOURNAL OF VIRAL HEPATITIS 2009 vol. 16(9) pp. 644-649 PRASAD L AND AL. Gene expression profile of Huh-7 cells expressing hepatitis C virus genotype 1b or 3a core proteins LIVER INTERNATIONAL 2009 vol. 29(5) pp. 661-669 PAZIENZA V, CLEMENT S, PUGNALE P, CONZELMANN S, PASCARELLA S, MANGIA A, NEGRO F Hepatitis C virus, steatosis and lipid abnormalities: clinical and pathogenic data. LIVER INTERNATIONAL 2009 vol. 29 pp. 26-37 NEGRO F, SANYAL AJ Peroxisome proliferator-activated receptors and hepatitis C virus-induced insulin resistance. PPAR RESEARCH 2009 vol. 2009 pp. 483485-483485 NEGRO F Pegylated interferon-alpha2a/ribavirin treatment of recurrent hepatitis C after liver transplantation. TRANSPLANT INFECTIOUS DISEASE : AN OFFICIAL JOURNAL OF THE TRANSPLANTATION SOCIETY 2009 vol. 11(1) pp. 33-39 DINGES S AND AL. Hepatitis C virus infection: molecular pathways to steatosis, insulin resistance and oxidative stress. VIRUSES-BASEL 2009 vol. 1(2) pp. 126-143 CLEMENT S, PASCARELLA S, NEGRO F Hepatitis C virus and type 2 diabetes. WORLD JOURNAL OF GASTROENTEROLOGY 2009 vol. 15(13) pp. 1537-1547 NEGRO F, ALAEI M Hepatitis C virus and glucose and lipid metabolism. DIABETES & METABOLISM 2008 vol. 34(6 Pt 2) pp. 692-700 ALAEI M, NEGRO F Mosaic inactivation of the serum response factor gene in the myocardium induces focal lesions and heart failure. EUROPEAN JOURNAL OF HEART FAILURE : JOURNAL OF THE WORKING GROUP ON HEART FAILURE OF THE EUROPEAN SOCIETY OF CARDIOLOGY 2008 vol. 10(7) pp. 635-645 GARY-BOBO G, PARLAKIAN A, ESCOUBET B, FRANCO CA, CLEMENT S, BRUNEVAL P, TUIL D, DAEGELEN D, PAULIN D, LI Z, MERICSKAY M Host- rather than virus-related factors reduce health-related quality of life in hepatitis C virus infection. GUT 2008 vol. 57(11) pp. 1597-1603 HELBLING B AND AL. Monocyte chemoattractant protein-1 secreted by adipose tissue induces direct lipid accumulation in hepatocytes. HEPATOLOGY 2008 vol. 48(3) pp. 799-807 CLEMENT S, JUGE-AUBRY CRISTIANA, SGROI A, CONZELMAN S, PAZIENZA VALERIO, PITTET-CUENOD B, MEIER CA, NEGRO F Hepatitis C viral kinetics in plasma and peripheral blood mononuclear cells during pegylated interferon-alpha2a/ribavirin therapy. JOURNAL OF HEPATOLOGY 2008 vol. 48(6) pp. 932-938 PUGNALE PAOLO, HERRMANN E, NEUMANN AU, PAWLOTSKY JM, SCHALM SW, FERRARI C, HOMBURGER Y, ZEUZEM S, NEGRO F Insulin resistance and response to therapy in patients infected with chronic hepatitis C virus genotypes 2 and 3. JOURNAL OF HEPATOLOGY 2008 vol. 48(1) pp. 28-34 POUSTCHI H, NEGRO F, HUI J, CUA IH, BRANDT LR, KENCH JG, GEORGE J Pioglitazone in chronic hepatitis C not responding to pegylated interferon-alpha and ribavirin. JOURNAL OF HEPATOLOGY 2008 vol. 49(2) pp. 295-298 OVERBECK K, GENNE D, GOLAY A, NEGRO F Virus-induced over-expression of protein phosphatase 2A inhibits insulin signalling in chronic hepatitis C. JOURNAL OF HEPATOLOGY 2008 vol. 49(3) pp. 429-440 BERNSMEIER C, DUONG FH, CHRISTEN V, PUGNALE PAOLO, NEGRO F, TERRACCIANO L, HEIM MH Active intravenous drug use during chronic hepatitis C therapy does not reduce sustained virological response rates in adherent patients. JOURNAL OF VIRAL HEPATITIS 2008 vol. 15(10) pp. 747-752 BRUGGMANN P, FALCATO L, DOBER S, HELBLING B, KEISER O, NEGRO F, MEILI D Connective tissue growth factor, steatosis and fibrosis in patients with chronic hepatitis C. LIVER INTERNATIONAL 2008 vol. 28(3) pp. 370-376 HORA C, NEGRO F, LEANDRO G, ONETA CM, RUBBIA BRANDT LAURA, MUELLHAUPT B, HELBLING B, MALINVERNI R, GONVERS JJ, DUFOUR JULIEN Steatosis in chronic hepatitis C: friend or foe? LIVER INTERNATIONAL 2008 vol. 28(3) pp. 294-296 NEGRO F HCV-specific T-cell response in relation to viral kinetics and treatment outcome (DITTO-HCV project) GASTROENTEROLOGY 2007 vol. 133 pp. 1132-1143 PILLI, M, ZERBINI, A, PENNA, A, ORLANDINI, A, LUKASIEWICZ, E, PAWLOTSKY, JM, ZEUZEM, S, SCHALM, SW, VON WAGNER, M, GERMANIDIS, G, LURIE, Y, ESTEBAN, JI, HAAGMANS, BL, HEZODE, C, LAGGING, M, NEGRO, F, HOMBURGER, Y, NEUMANN, AU, FERRARI, C, MISSALE, G The hepatitis C virus core protein of genotypes 3a and 1b downregulates insulin receptor substrate 1 through genotype-specific mechanisms HEPATOLOGY 2007 vol. 45 pp. 1164-1171 PAZIENZA, V, CLEMENT, S, PUGNALE, P, CONZELMAN, S, FOTI, M, MANGIA, A, NEGRO, F Cohort Profile: the Swiss Hepatitis C Cohort Study (SCCS) INTERNATIONAL JOURNAL OF EPIDEMIOLOGY 2007 vol. 36(4) pp. 731-737 PRASAD LEONIE, MASSEREY SPICHER VIRGINIE, ZWAHLEN MARCEL, RICKENBACH MARTIN, HELBING BEAT, NEGRO FRANCESCO, SWISS HEPATITIS C COHORT STU.G In vitro antiviral activity of SCH446211 (SCH6), a novel inhibitor of the hepatitis C virus NS3 serine protease JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 2007 vol. 59 pp. 51-58 LIU, R, ABID, K, PICHARDO, J, PAZIENZA, V, INGRAVALLO, P, KONG, R, AGRAWAL, S, BOGEN, S, SAKSENA, A, CHENG, KC, PRONGAY, A, NJOROGE, FG, BAROUDY, BM, NEGRO, F Hepatitis C virus: The viral way to fatty liver JOURNAL OF HEPATOLOGY 2007 vol. 46 pp. 985-987 CLEMENT S, NEGRO F Impact of hepatic steatosis on viral kinetics and treatment outcome during antiviral treatment of chronic HCV infection JOURNAL OF VIRAL HEPATITIS 2007 vol. 14 pp. 29-35 WESTIN J, LAGGING M, DHILLON AP, NORKRANS G, ROMERO AI, PAWLOTSKY JM, ZEUZEM S, SCHALM SW, VERHEIJ-HART E, NEGRO F, MISSALE G, NEUMANN AU, HELLSTRAND K Hepatitis C virus induces proteolytic cleavage of sterol regulatory element binding proteins and stimulates their phosphorylation via oxidative stress JOURNAL OF VIROLOGY 2007 vol. 81(15) pp. 8122-8130 WARIS GULAM, FELMLEE DANIEL JEFFREY, NEGROS FRANCESCO, SIDDIQUI ALEEM Notre ami...le café REVUE MÉDICALE SUISSE 2007 vol. 3(123) pp. 1931- HADENGUE A, NEGRO F Peroxisome proliferator-activated receptor-alpha and -gamma mRNA levels are reduced in chronic hepatitis C with steatosis and genotype 3 infection ALIMENTARY PHARMACOLOGY & THERAPEUTICS 2006 vol. 23(1) pp. 107-1114 DE GOTTARDI A, PAZIENZIA V, PUGNALE P, BRUTTINI F, RUBBIA-BRANDT L, JUGE-AUBRY CE, MEIER CA, HADENGUE A, NEGRO F Review article: management of patients with chronic hepatitis C virus infection and 'normal' alanine aminotransferase activity ALIMENTARY PHARMACOLOGY & THERAPEUTICS 2006 vol. 24 pp. 1133-1149 ZEUZEM S, ALBERTI A, ROSENBERG W, MARCELLIN P, DIAGO M, NEGRO F, PRATI D, PUOTI C, ROBERTS SK, SHIFFMAN ML Relationship between steatosis, inflammation, and fibrosis in chronic hepatitis C: a meta-analysis of individual patient data GASTROENTEROLOGY 2006 vol. 130(6) pp. 1636-1642 GIOACCHINO LEANDRO ET AL. Steatosis in chronic hepatitis C: why does it really matter? GUT 2006 vol. 55(1) pp. 123-130 ASSELAH T, RUBBIA-BRANDT L, MARCELLIN P, NEGRO F IP-10 predicts viral response and therapeutic outcome in difficult-to-treat patients with HCV genotype 1 infection HEPATOLOGY 2006 vol. 44 pp. 1617-1625 LAGGING M, ROMERO AI, WESTIN J, NORKRANS G, DHILLON AP, PAWLOTSKY JM, ZEUZEM S, VON WAGNER M, NEGRO F, SCHALM SW, HAAGMANS BL, FERRARI C, MISSALE G, NEUMANN AU, VERHEIJ-HART E, HELLSTRAND K Insulin resistance and HCV: Will new knowledge modify clinical management? JOURNAL OF HEPATOLOGY 2006 vol. 45 pp. 514-519 NEGRO FRANCESCO The paradox of Epstein-Barr virus-associated hepatitis JOURNAL OF HEPATOLOGY 2006 vol. 44 pp. 839-841 NEGRO FRANCESCO The paradox of Epstein-Barr virus-associated hepatitis. JOURNAL OF HEPATOLOGY 2006 vol. 44(5) pp. 839-841 NEGRO F Interferon (IFN)-gamma-inducible protein-10: Association with histological results, viral kinetics, and outcome during treatment with pegylated IFN-alpha 2a and ribavirin for chronic hepatitis C virus infection JOURNAL OF INFECTIOUS DISEASES 2006 vol. 194 pp. 895-903 ROMERO AI, LAGGING M, WESTIN J, DHILLON AP, DUSTIN LB, PAWLOTSKY JM, NEUMANN AU, FERRARI C, MISSALE G, HAAGMANS BL, SCHALM SW, ZEUZEM S, NEGRO F, VERHEIJ-HART E, HELLSTRAND K Real-time multiplex PCR assay to quantify hepatitis C virus RNA in peripheral blood mononuclear cells JOURNAL OF VIROLOGICAL METHODS 2006 vol. 133 pp. 195-204 PUGNALE P, LATORRE P, ROSSI C, CROVATTO K, PAZIENZA V, DE GOTTARDI A, NEGRO F Optimized vaccination regimen linked to exhaustive screening approaches identifies 2 novel HLA-B7 restricted epitopes within hepatitis C virus NS3 protein MICROBES AND INFECTION : A JOURNAL ON INFECTIOUS AGENTS AND HOST DEFENSES 2006 vol. 8(9-10) pp. 2432-2441 MARTIN PERRINE ET AL. The tragedy of (unpublished) errors NATURE CLINICAL PRACTICE GASTROENTEROLOGY & HEPATOLOGY 2006 vol. 3 pp. 238-239 GENTA RM, NEGRO F Construction and characterization of infectious intragenotypic and intergenotypic hepatitis C virus chimeras PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2006 vol. 103 pp. 7408-7413 PIETSCHMANN T, KAUL A, KOUTSOUDAKIS G, SHAVINSKAYA A, KALLIS S, STEINMANN E, ABID K, NEGRO F, DREUX M, COSSET FL, BARTENSCHLAGER R Le point sur le traitement antiviral de l'hépatite B chronique REVUE MEDICALE DE LA SUISSE ROMANDE 2006 vol. 2(77) pp. 1981-1985 NEGRO FRANCESCO, HADENGUE ANTOINE Les enfants de Zanzibar, l'hepcidine et le fer REVUE MEDICALE DE LA SUISSE ROMANDE 2006 vol. 2(77) pp. 1947-1948 HADENGUE ANTOINE, NEGRO FRANCESCO Mechanisms and significance of liver steatosis in hepatitis C virus infection WORLD JOURNAL OF GASTROENTEROLOGY 2006 vol. 12 pp. 6756-6765 NEGRO FRANCESCO Molecular evidence that the hepatitis C virus replicates in the oral mucosa JOURNAL OF HEPATOLOGY 2002 vol. 37 pp. 364-369 CARROZZO M, QUADRI R, LATORRE P, PENTENERO M, PAGANIN S, BERTOLUSSO G, GANDOLFO S, NEGRO F Immunological and virological effects of ribarivin in hepatitis C after liver transplantation TRANSPLANTATION 2002 vol. 73 pp. 373-378 QUADRI R, GIOSTRA E, ROSKAMS T, PAWLOTSKY JM, MENTHA G, RUBBIA-BRANDT L, PERRIN L, HADENGUE A, NEGRO F Nosocomial outbreak of multiple bloodborne viral infections. JOURNAL OF INFECTIOUS DISEASES 2001 vol. 184 pp. 369-372 YERLI S, QUADRI R, NEGRO F, BARBE KP, CHESEAUX JJ, BURGISSER P, SIEGRIST CA, PERRIN L Hepatocyte steatosis is a cytopathic effect of hepatitis C virus genotype 3 JOURNAL OF HEPATOLOGY 2000 vol. 33 pp. 106-115 RUBBIA-BRANDT L, QUADRI R, ABID K, GIOSTRA E, MALE PJ, MENTHA G, SPAHR L, ZARSKI JP, BORISCH B, HADENGUE A, NEGRO F Detection of genomic- and minus-strand of hepatitis C virus RNA in the liver of chronic hepatitis C patients by strand-specific semi-quantitative RT-PCR HEPATOLOGY 1999 vol. 29 pp. 536-542 NEGRO F, KRAWCZYNSKI K, QUADRI R, RUBBIA-BRANDT L, MONDELLI M, ZARSKI JP, HADENGUE A Extranodal lymphomas associated with hepatitis C virus infection AMERICAN JOURNAL OF CLINICAL PATHOLOGY 1998 vol. 109 pp. 600-609 ASCOLI V, LO COCO F, ARTINI M, LEVRERO M, MARTELLI M, NEGRO F Domaines de recherche HEPATITE VIRALE C CHRONIQUE VIRUS DE L'HEPATITE C PATOGENESE DES MALADIES INFECTIEUSES STEATOSE HEPATIQUE TRAITEMENT ANTIVIRAL |