|
|
|||||||
|
|
|
|
|
|
|
|
|
|||||||
|
BASE DE DONNÉES-RECHERCHE | |
GROUPE DE RECHERCHE CLINIQUE ORIENTEE BIOLOGIE | ||||
|
|||||||
|
Nicolas DEMAUREX | CV du Chef de Groupe | Sujet de Recherche | Composition du Groupe | |||
|
|
|
|
|
Coordonnées du groupe
Pr. N. DEMAUREX CMU/ Dpt PHYM Rue Michel-Servet 1 1211 Genève 4 Suisse Nicolas.Demaurex@unige.ch Tél.: 022 379 53 99 Fax: 022 379 54 02 Site web du groupe / département Actualités Groupe recherche personnel Publication Commentaires Pages générées le 23.02.2012 |
Sujet de recherche
|
Publications du groupe
|
Domaines de recherche
Signaux intracellulaires
Nous étudions le rôle du calcium et du pH sur les fonctions cellulaires, ainsi que les mécanismes contrôlant l¿activité de ces ions dans les différents compartiments de la cellule. Alors que le
maintien d¿un pH optimal est essentiel pour la plupart des réactions biologiques, des élévations locales de calcium peuvent activer des fonctions aussi diverses que la contraction musculaire ou la
transmission synaptique. Nous utilisons des protéines fluorescentes dérivées de la calmoduline pour mesurer les signaux calciques à l¿intérieur des organites intracellulaires. Ces sondes peuvent être
introduites, par des techniques de biologie moléculaire, soit dans le cytosol, soit dans le noyau, les mitochondries ou le réticulum endoplasmique.Cette approche est utilisée pour étudier le rôle de la
calréticuline, la principale protéine stockant le calcium à l¿intérieur du réticulum endoplasmique. A cette fin, nous mesurons les signaux calciques dans le cytosol et dans le réticulum de cellules dont le
niveau d¿expression de calréticuline
peut être modulé de façon spécifique. Le rôle du calcium nucléaire dans l¿activation de gènes est étudié à l¿aide de protéines fluorescentes, placées sous le
contrôle d¿un promoteur d¿intérêt. Une
concentration stable de calcium est établie dans le noyau par microinjection, et l¿activation de la transcription est mesurée par fluorescence.Nous étudions également
les mécanismes de régulation du pH intracellulaire. Le transport conductif de protons par la NADPH oxydase, une enzyme qui catalyse le transfert d¿électrons à travers la membrane plasmique, est
étudié par des techniques d¿électrophysiologie et de fluorescence.
Publications du groupe Regulation of plasma membrane calcium fluxes by mitochondria BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 2009 vol. 1787(11) pp. 1383-1394 DEMAUREX N, POBURKO D, FRIEDEN M Amiloride derivatives induce apoptosis by depleting ER Ca2+ stores in vascular endothelial cells BRITISH JOURNAL OF PHARMACOLOGY 2009 vol. 156(8) pp. 1296-1304 PARK KS, POBURKO D, WOLLHEIM CB, DEMAUREX N Local and global calcium signals associated with the opening of neuronal alpha 7 nicotinic acetylcholine receptors CELL CALCIUM 2009 vol. 45(2) pp. 198-207 GILBERT D, LECCHI M, ARNAUDEAU S, BERTRAND D, DEMAUREX N Mitochondrial Regulation of Sarcoplasmic Reticulum Ca2+ Content in Vascular Smooth Muscle Cells CIRCULATION RESEARCH 2009 vol. 104(1) pp. 104-112 POBURKO D, LIAO CH, VAN BREEMEN C, DEMAUREX N Matrix alkalinization: a novel mitochondrial signal for sustained pancreatic beta-cell activation EMBO JOURNAL 2009 vol. 28(4) pp. 417-428 WIEDERKEHR A, PARK KS, DUPONT O, DEMAUREX N, POZZAN T, CLINE GW, WOLLHEIM CB STIM1-and Orai1-dependent Store-operated Calcium Entry Regulates Human Myoblast Differentiation JOURNAL OF BIOLOGICAL CHEMISTRY 2009 vol. 284(8) pp. 5370-5380 DARBELLAY B, ARNAUDEAU S, KONIG S, JOUSSET H, BADER CR, DEMAUREX N, BERNHEIM L Reactive oxygen species are NOXious for neurons NATURE NEUROSCIENCE 2009 vol. 12(7) pp. 819-820 DEMAUREX N, SCORRANO L STIM1-induced precortical and cortical subdomains of the endoplasmic reticulum PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2009 vol. 106(46) pp. 19358-19362 ORCI L, RAVAZZOLA M, LE COADIC M, SHEN WW, DEMAUREX N, COSSON P A Revolving Door for Calcium SCIENCE 2009 vol. 326(5949) pp. 57-58 DEMAUREX N, POBURKO D Endoplasmic reticulum Ca2+ measurements reveal that the cardiac ryanodine receptor mutations linked to cardiac arrhythmia and sudden death alter the threshold for store-overload-induced Ca2+ release. BIOCHEMICAL JOURNAL 2008 vol. 412(1) pp. 171-178 JONES PP, JIANG D, BOLSTAD J, HUNT DJ, ZHANG L, DEMAUREX N, CHEN SR Evidence for a receptor-activated Ca2+ entry pathway independent from Ca2) store depletion in endothelial cells. CELL CALCIUM 2008 vol. 43(1) pp. 83-94 JOUSSET H, MALLI R, GIRARDIN N, GRAIER WF, DEMAUREX N, FRIEDEN M Dual effect of cell-cell contact disruption on cytosolic calcium and insulin secretion. ENDOCRINOLOGY 2008 vol. 149(5) pp. 2494-2505 JAQUES F, JOUSSET H, TOMAS A, PROST AL, WOLLHEIM CB, IRMINGER JC, DEMAUREX N, HALBAN P Selective actions of mitochondrial fission/fusion genes on metabolism-secretion coupling in insulin-releasing cells. JOURNAL OF BIOLOGICAL CHEMISTRY 2008 vol. 283(48) pp. 33347-33356 PARK KS, WIEDERKEHR A, KIRKPATRICK C, MATTENBERGER Y, MARTINOU JC, MARCHETTI A, DEMAUREX N, WOLLHEIM CB NOX5 is expressed at the plasma membrane and generates superoxide in response to protein kinase C activation BIOCHIMIE 2007 vol. 89 pp. 1159-1167 SERRANDER L, JAQUET V, BEDARD K, PLASTRE O, HARTLEY O, ARNAUDEAU S, DEMAUREX N, SCHLEGEL W, KRAUSE KH STIM1 knockdown reveals that store-operated Ca2+ channels located close to sarco/endoplasmic Ca2+ ATPases (SERCA) pumps silently refill the endoplasmic reticulum JOURNAL OF BIOLOGICAL CHEMISTRY 2007 vol. 282 pp. 11456-11464 JOUSSET H, FRIEDEN M, DEMAUREX N Role of nucleotides and phosphoinositides in the stability of electron and proton currents associated with the phagocytic NADPH oxidase BIOCHEMICAL JOURNAL 2006 vol. 400 pp. 431-438 PETHEO GL, GIRARDIN NC, GOOSSENS N, MOLNAR GZ, DEMAUREX N GABA regulates dendritic growth by stabilizing lamellipodia in newly generated interneurons of the olfactory bulb JOURNAL OF NEUROSCIENCE 2006 vol. 26 pp. 12956-12966 GASCON E, DAYER AG, SAUVAIN MO, POTTER G, JENNY B, DE ROO M, ZGRAGGEN E, DEMAUREX N, MULLER D, KISS JZ Voltage- and NADPH-dependence of electron currents generated by the phagocytic NADPH oxidase. BIOCHEMICAL JOURNAL 2005 vol. 388 pp. 485-491 PETHEO GABOR, DEMAUREX NICOLAS Calcium measurements in organelles with Ca2+-sensitive fluorescent proteins. CELL CALCIUM 2005 vol. 38 pp. 213-222 DEMAUREX NICOLAS Subplasmalemmal mitochondria modulate the activity of plasma membrane Ca2+-ATPases. JOURNAL OF BIOLOGICAL CHEMISTRY 2005 vol. 280 pp. 43198-43208 FRIEDEN MAUD, ARNAUDEAU SERGE, CASTELBOU CYRIL, DEMAUREX NICOLAS The role of mitochondria for Ca2+ refilling of the endoplasmic reticulum JOURNAL OF BIOLOGICAL CHEMISTRY 2005 vol. 280(13) pp. 12114-12122 MALLI R, FRIEDEN MAUD, TRENKER M, GRAIER WF Endosome-to-cytosol transport of viral nucleocapsids. NATURE. CELL BIOLOGY 2005 vol. 7 pp. 653-664 LE BLANC ISABELLE ET AL. Electron and proton transport by NADPH oxidases. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON. SERIES B: BIOLOGICAL SCIENCES 2005 vol. 360 pp. 2315-2325 DEMAUREX NICOLAS, PETHEO GABOR Gelsolin mediates calcium-dependent disassembly of Listeria actin tails PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2005 vol. 102 pp. 1921-1926 LARSON LAURA ET AL. HIV-1 trafficking to the dendritic cell-T-cell infectious synapse uses a pathway of tetraspanin sorting to the immunological synapse. TRAFFIC 2005 vol. 6 pp. 488-501 GARCIA EDUARDO ET AL. ARF1 regulates Nef-induced CD4 degradation CURRENT BIOLOGY : CB 2004 vol. 14(12) pp. 1056-1064 FAURE JULIEN, STALDER R, BOREL CHRISTELLE, SOBO KOMLA, PIGUET VINCENT, DEMAUREX NICOLAS, GRUENBERG JEAN, TRONO DIDIER Calcium homeostasis during mitochondrial fragmentation and perinuclear clustering induced by hFis1 JOURNAL OF BIOLOGICAL CHEMISTRY 2004 vol. 279 pp. 22704-22714 FRIEDEN MAUD, JAMES D, CASTELBOU CYRIL, DANCKAERT A, MARTINOU J-C, DEMAUREX NICOLAS Measurements of the free luminal ER Ca(2+) concentration with targeted "cameleon" fluorescent proteins. CELL CALCIUM 2003 vol. 34(2) pp. 109-119 DEMAUREX N, FRIEDEN M Intracellular transport of calcium from plasma membrane to mitochondria in adrenal H295R cells: implication for steroidogenesis. ENDOCRINOLOGY 2003 vol. 144 pp. 4575-4585 LALEVÉE N, RESIN V, ARNAUDEAU S, DEMAUREX N, ROSSIER MF Mitochondrial efficiently buffer subplasmalemmal Ca2+ elevation during agonist stimulation JOURNAL OF BIOLOGICAL CHEMISTRY 2003 vol. 278(12) pp. 10807-10815 MALLI R, FRIEDEN M, OSIBOW K, GRAIER WF Sustained Ca2+ transfer across mitochondria is Essential for mitochondrial Ca2+ buffering, sore-operated Ca2+ entry, and Ca2+ store refilling JOURNAL OF BIOLOGICAL CHEMISTRY 2003 vol. 278 pp. 44769-44779 MALLI R, FRIEDEN M, OSIBOW K, ZORATTI C, MAYER M, DEMAUREX N, GRAIER WF A tyrosine-based sorting signal is involved in connexin43 stability and gap junction turnover. JOURNAL OF CELL SCIENCE. 2003 vol. 116 pp. 2213-2222 THOMAS MA, ZOSSO N, SCERRI I, DEMAUREX N, CHANSON M, STAUB O Interactions between electron and proton currents in excised patches from human eosinophils. JOURNAL OF GENERAL PHYSIOLOGY 2003 vol. 122 pp. 713-726 PETHEÖ GL, SPAT A, MATURANA A, DEMAUREX N Apoptosis--the calcium connection SCIENCE 2003 vol. 300 pp. 65-67 DEMAUREX N, DISTELHORST C Molecular and functional characterization of a new X-linked chronic granulomatous disease variant (X91+) case with a double missense mutation in the cytosolic gp91phox C-terminal tail BIOCHIMICA ET BIOPHYSICA ACTA : INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND BIOPHYSICS. 2002 vol. 1586 pp. 316-330 STASIA MARIE JOSÉ, LARDY BERNARD, MATURANA A, ROUSSEAU PASCALE, MARTEL CÉCILE, BORDIGONI PIERRE, DEMAUREX N, MOREL FRANÇOISE Charybdotoxin-sensitive small conductance K(Ca) channel activated by bradykinin and substance P in endothelial cells BRITISH JOURNAL OF PHARMACOLOGY 2002 vol. 136 pp. 1201-1209 SOLLINI M, FRIEDEN M, BENY JL Tissue-specific expression of human lipoprotein lipase in the vascular system affects vascular reactivity in transgenic mice BRITISH JOURNAL OF PHARMACOLOGY 2002 vol. 135 pp. 143-154 ESENABHALU VICTOR E, CERIMAGIC MIRZA, MALLI ROLAND, OSIBOW KARIN, LEVAK-FRANK SANJA, FRIEDEN M, SATTLER WOLFGANG, KOSTNER GERHARD M, ZECHNER RUDOLF, GRAIER WOLFGANG F Calreticulin differentially modulates calcium uptake and release in the endoplasmic reticulum and mitochondria JOURNAL OF BIOLOGICAL CHEMISTRY 2002 vol. 277 pp. 46696-46705 ARNAUDEAU S, FRIEDEN M, NAKAMURA KIMITOSHI, CASTELBOU C, DEMAUREX N Spontaneous calcium oscillations control c-fos transcription via the serum response element in neuroendocrine cells JOURNAL OF BIOLOGICAL CHEMISTRY 2002 vol. 277 pp. 39713-39721 MATURANA A, VAN HAASTEREN G, PIUZ I, CASTELBOU C, DEMAUREX N, SCHLEGEL W Nox family nadph oxidases: Do they have built-in proton channels? JOURNAL OF GENERAL PHYSIOLOGY 2002 vol. 120 pp. 781-786 MATURANA A, KRAUSE KH, DEMAUREX N The dendritic cell-specific adhesion receptor DC-SIGN internalizes antigen for presentation to T cells JOURNAL OF IMMUNOLOGY 2002 vol. 168 pp. 2118-2126 ENGERING A, GEIJTENBEEK TB, VAN VLIET SJ, WIJERS M, VAN LIEMPT E, DEMAUREX N, LANZAVECCHIA A, FRANSEN J, FIGDOR CG, PIGUET V, VAN KOOYK Y Subplasmalemmal endoplasmic reticulum controls K(Ca) channel activity upon stimulation with a moderate histamine concentration in a human umbilical vein endothelial cell line JOURNAL OF PHYSIOLOGY 2002 vol. 540 pp. 73-84 FRIEDEN M, MALLI ROLAND, SAMARDZIJA MARIANA, DEMAUREX N, GRAIER WOLFGANG F Measurement of intracellular Ca2+ concentration METHODS IN CELL BIOLOGY 2002 vol. 70 pp. 453-474 DEMAUREX N, ARNAUDEAU S, OPAS MICHAL pH Homeostasis of cellular organelles NEWS IN PHYSIOLOGICAL SCIENCES 2002 vol. 17 pp. 1-5 DEMAUREX N Domaines de recherche |